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To characterize in more detail the structural changes induced by GdnHCl, we have investigated the accessibility of thiol groups of GR (which contains six Cys residues per subunit) to modification with DTNB (Fig government grant and loan program .
The original IR or fluorescence data were normalised by the corresponding intensity of the spectra recorded al 30°C government grant and loan program .
In contrast, the recombinant protein unfolds via an intermediate government grant and loan program .
The use of theories based on perturbations around the Θ state is only valid in the limit T [arrow right] Θ or small N government grant and loan program .
or 350 nm, after excitation at 280 and 295 nm, with a scan rate of 30 K/h government grant and loan program .
Study of the membrane permeability of a paramagnetic complex on single cells by NMR microscopy government grant and loan program .
Identification of nanosecond-timescale internal motionThe presence of nanosecond-timescale motion can be directly identified from a plot of the normalized ratio of R^sub 1^ values measured at two magnetic fields (RT^sup n^^sub 1^) versus NOE (22) government grant and loan program .
Preliminary activity studies show that addition of Gα^sub s^(GDP) deactivates the activated PLCβ-Gβγ complex similar to the behavior seen for the full length enzyme government grant and loan program .
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The mixture were then injected subcutaneously into BALB/c female mice (4 weeks of age) government grant and loan program .
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