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This limit is of interest in light of data that suggest the presence of residual structure even under strongly denaturing conditions
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Furthermore, the cooling curve of the apoA-2:DMPC sample shows partial protein refolding below 80°C, followed by additional refolding between 32 and 24°C, i
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Hence, the 4WJ ribozyme is unique in its ability to fold into a structure with arms A and B in close proximity, similar to the native ribozyme, even in the absence of specific loop-loop docking interactions
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Because the two loop-carrying arms are in close proximity, it is likely that the quasi-docked conformation facilitates the formation of tertiary interactions between loops A and B
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