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. Conformational constraints for amyloid formation: the importance of being unfolded
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. As already mentioned above, there is indication from previous studies on several α-amylases that a structural unfolding is followed by an irreversible process (14,22-25)
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. Use of fluorescence decay times of 8-ANS-protein complexes to study the conformational transitions in proteins which unfold through the molten globule state
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. The Principles of Nuclear Magnetism
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. Experimentally these states have been principally observed in colloidal systems although it has been shown that there are strong analogies between the behavior of colloidal and concentrated globular protein solutions (2)
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. We have found that for membranes composed of lipids with electrically neutral or negatively charged head groups, no change in surface charge could be detected at neutral pH from 1 to 2000 bar
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. The free energy of binding, however, is small due to a remarkable entropy/enthalpy compensation
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. To estimate errors, several independent simulations could be performed and compared
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. Three curing temperatures (150, 175, and 200°C) and three curing times (1, 2, and 3 minutes) were considered
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. The intensity changes of the various fluorophores upon heating could be classified into three distinct stages
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. It is also noteworthy that the observed scaling behavior is valid for a range of chain lengths that includes short chains (11)
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. The number and locations of nonnative local contacts that are to be broken determine the sets of spatial contacts that are formed upon folding
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. For comparison, the absolute contact orders of the native-state counterparts are also shown
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.5°C) with respect to its native counterpart
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. ThT binds to oligomers and shows large amplitude in the slow second phase in response to formation of spherical aggregates and protofibrils, well in accordance with its reported amyloid specificity (Naiki et al
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