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. Information on the dynamics of protein hydration may be obtained from ^sup 17^O magnetic relaxation dispersion measurements, which indicate that water molecules at protein surfaces are highly mobile (66,67)
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. 5 h at 75°C) or at higher temperatures (30 min at 95°C) leads to an increased population of large multilamellar liposomes (Fig
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. When key stabilizing interactions succeed in reducing the dimensionality of the search space more drastically, much higher values can result (from differentiation of the higher-dimensional analogs of Eq
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. Identification of individual HIV type 1 gp120 amino acids important for CD4 receptor binding
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. Additionally, the z-test indicated that the magnitude of the correlation coefficients between the SLSI and the PCS-12 did not vary significantly across genders or the three respective class years
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. Dielectric Behavior of Biological Molecules in Solution
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.8), 150 mM sodium chloride, 15% glycerol, 1 mM Ca^sup 2+^/Mn^sup 2+^, and 200 mM lactose)
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. In support of this notion, the intratracheal instillation of apoptotic Jurkat T cells in the presence of TNF-[alpha] (an inhibitor of efferocytosis) results in the development of a transient emphysema-like injury pattern (V
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. In this work we report a case study on five homologous α-amylases aiming to elucidate the impact of protein aggregation and conformational scrambling of the unfolded states on fluorescence emission
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.MATERIALS AND METHODSEnzyme purification and characterizationHuBChE was purified to homogeneity from outdated human plasma according to an improved protocol based on that of Grunwald et al
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.Data analysisConversion of the apparent K(d) obtained on membrane surfaces to the K(d) that would be obtained if the proteins were freely diffusing has been previously treated by assuming that the proteins are confined to interact within a reduced surface volume (ν) that is equal to the surface area of the membrane multiplied by the distance from the membrane surface into the aqueous phase where the proteins interact, which is taken to be 50 nm
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. coli lac repressor-operator interaction: kinetic measurements and conclusions
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., 1996) and the extension or stretching of the coiled-coil gp41, leading to the insertion of the membrane fusion peptide into the host membrane, which induces fusion of the viral and host membranes (Chan and Kim, 1998)
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