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Intermediates in the folding reactions of small proteins
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Conformational stability of globular proteins
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We use our results to resolve between conflicting proposals for structure in ensembles for highly denatured states
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Converse to observations in other EI proteins by DSC (6) and CD (52)
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Reversible equilibrium unfolding of triosephosphate isomerase from Trypanosoma cruzi in guanidinium hydrochloride involves stable dimeric and monomeric intermediates
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In contrast, RhoA induces the formation of stress fibers, focal adhesions, and cell spreading
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Dynamics on micro- to millisecond timescaleThree different approaches were used to determine which CaM residues in the CaM:SEF2-1mp complex are affected by conformational exchange
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Supercoiling in closed circular DNA: dependence upon ion type and concentration
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However, the off-rate of the association was too slow to be physiologically relevant (~2 min), suggesting that other cellular processes might serve to reduce the off-rate
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We determined stage-specific fecundity in black rockfish for females with unfertilized yolked oocytes (n=184) and fertilized eggs (n=85)
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93 in the current study
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.MATERIALS AND METHODSSample preparationSEF2-1mp mimics the DNA- and CaM-binding region of the bHLH transcription factor SEF2-1
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