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, 1978; Bolotina, 1987) and fluctuating tertiary structure
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the fluorescence was significantly affected by the appearance of serious aggregation
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Conformational properties of unfolded proteins
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However, quenching experiments and proteolysis patterns suggest conformational changes in the presence of PEP; furthermore, the thermal stability of EI^sup SC^ was modified depending on the effector added
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In short, apoptotic cells must express clear-cut "eat-me" signals (Fig 2, top, A)
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J Exp Med 2000; 191:515-528(18) Okazawa H, Motegi S, Ohyama N, et al
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Lower protein concentrations and smaller heating rates lead to larger red shifts upon thermal unfolding, indicating that under these conditions the protein aggregation is less pronounced
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We examined two single point cavity-forming mutants, I7S and F110S, whose internal cavities of 40 and 100 [Angstrom], respectively, appear to be fully hydrated (18), and the double mutant C3A/C26A in which the disulfide bridge linking strands β1 and β2 has been severed
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In Advances in Chemical Physics, Vol
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15 mN for eutectic Sn-Pb solder and 1
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Total length (TL), body length (=standard length), head length (HL), predorsal length (PDL), and preanal length (PAL) were measured to the nearest 1 mm
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Segments or regions that are directly involved in intermolecular interactions or that are indirectly affected upon binding: residue F43 of CD4 (code: F43) and the residues of gp120 forming a cavity to hold F43 (code: F43cav), residues of gp120 that form intermolecular hydrogen bonds (code: interHb) and their counterparts in CD4 (code: interHb); see Fig
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Time-resolved FRET (trFRET) measurements were subsequently used to resolve and quantify the equilibrium distribution of extended and docked ribozyme conformers (N
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, 1999) have also been demonstrated
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Energy transfer: a spectroscopic ruler
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