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. That discovery led to Endothil-CR
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. With respect to the location of tryptophan residues we can roughly distinguish between two different classes of proteins among the α-amylases
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. In conclusion, from examining azurin mutants of lower thermodynamic stability and greater internal flexibility than the WT, it was found that the perturbation of the native fold in the frozen state, evidenced by the capacity to bind ANS, is enhanced by the plasticity of the globular structure and correlates with the lability to freeze-thawing
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. The same figure also shows the S^sup 2^^sub f^ contours when an additional nanosecond-timescale internal motion is present with time constant τ^sub is^ = 2 ns and order parameter S^sup 2^^sub s^ = 0
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. For the residues with significant exchange broadening, specific k^sup -1^^sub ex^ values were derived (Table 1)
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.Here τ^sub 1^, τ^sub 2^ are the exponential relaxation times, A^sub 1^, A^sub 2^ are the amplitudes, and A^sub 0^ is the value of Θ^sub 222^ at t [arrow right] ∞
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