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. The average shape of a denatured protein is that of a prolate ellipsoid We computed the ensemble-averaged asphericity values for each of the 23 sequences in the EV limit and the resultant data are shown as cross marks in Fig
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. Thus, we confined our study to a cell system with intracellular compartments larger than the smallest voxel dimension we could realistically obtain
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. Suzanne ScarlataDepartment of Physiology & Biophysics, State University of New York at Stony Brook, New York 11794-8661Submitted November 4, 2004, and accepted for publication January 18, 2005
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. After the buffer baseline subtraction, the data were normalized to protein concentration
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. Protein tyrosine kinases; from inhibitors to useful drugs158829384XProtein tyrosine kinases; from inhibitors to useful drugs
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.Titration of reactive thiol groupsThis was performed by following the increase in 2-nitro-5-thiobenzoic acid (TNB) absorbance at 412 nm, according to Collier (1973)
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. Of particular interest is the value of R^sub o^, which is related to the persistence length and also provides a measure of the goodness of the solvent because R^sup 3^^sub o^ quantifies the average volume per residue set aside by the chain for interactions with the surrounding solvent (38,41)
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. When attempting to elucidate a plausible mechanism of formation of these early, yet relatively thick fibrils, it must be taken into account that the fibrils' ends are not frayed, i
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