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Has everyone gone crazy? Well
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The influence of high hydrostatic pressure on structure, function and assembly of proteins and protein complexes
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Sample preparation Before sample preparation, all protein stocks were dialyzed against 2 mM Hepes buffer, pH 7
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I7S and F110S are characterized by an internal cavity caused by the substitution of bulky Ile and Phe with smaller Ser, whereas in C3A/C26A the disulride bridge linking strands β1 and β2 has been severed
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The latter feature shows that weak binding sites, similar to those induced in azurin by the destabilizing salt KSCN (12), are common with proteins under these conditions
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In solutions of polyethylene glycol and salt, DNA undergoes polymer- and salt-induced (PSI) condensation (Post and Zimm, 1979; Grosberg et al
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In addition ANS shows a minor slow second phase in response to formation of larger assemblies (spherical aggregates and protofibrils)
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In contrast to the large effects of pressure on the membrane interior, pressure does not appear to affect the membrane surface charge and has little effect on surface hydration (Rebecchi et al
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5, in which we assume that the transition state energy depends on denaturant concentration in the same way as the gutter energy, for the sake of illustration
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Several reviews have been published on the subject, notably a 2004 article by Peter J
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The GROMACS program package (Lindahl et al
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By combining the trajectories of these states, however, more information can be obtained
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The mixture were then injected subcutaneously into BALB/c female mice (4 weeks of age)
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Table 1Characteristics of Sparidae distributed in Japan
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This latest report is the third in a series of documents exploring ways to ensure the safety of the U
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Explain that this line of research is called social neuroscience
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ANSWERS TO QUIZ QUESTIONS:1
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The monkeys that experienced a stressful environment
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. In experiments aimed at detecting the burial of the aromatic residues upon binding to PEP and/or Mg^sup 2+^, the concentration of EI^sup sc^ was 4 µM at pH 7
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