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. This work was supported in part by a grant-in-aid for the 21st Century COE "Center for Diversity and Universality in Physics" from the Ministry of Education, Culture, Sports, Science and Technology of Japan
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. Despite the limited sampling time of 10 ns, the theoretically derived changes in configurational entropy are in fair agreement with the experimentally determined data
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. To look at the generality of the phenomenon, ANS binding experiments were carried out on seven new proteins, namely, monomers (bovine serum albumin (BSA), α-amylase, and β-galactosidase (β-gal)), a dimer (alcohol dehydrogenase from horse liver (LADH)), and tetramers (alcohol dehydrogenase from yeast (YADH), lactic dehydrogenase (LDH), and aldolase (ALD))
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. Upon peptide binding, the flexibility of side chains located in the binding sites is redistributed
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. Residues 14, 19, 51, 55, 76, 92, and 118 show a relatively strong decrease in exchange rate upon change in temperature (f = 0
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