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. All the experiments were done in a Jobin Yvon Horiba fluorometer (Jobin Yvon Spex, Cedex, France) in a 1-cm water- jacketed cell using a protein concentration of 1 µM and ANS concentration of 50 µM
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. Here, q is in units of inverse wavelength
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. A first-principles reassessment of the source of this empirical correlation is mandated
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. In an attempt to correlate affinities and n values to specific aspects of the macromolecule, we observe that all mutants are less stable than the WT by a comparable amount, the denaturation-free energy change, ΔG° at 20°C, decreasing from 6-7 to ~3 kcal/mole (20,27)
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. Gradient, high-resolution, magic angle spinning ^sup 1^H nuclear magnetic resonance spectroscopy of intact cells
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. , it weighs contributions from all atoms), it cannot be ruled out a priori that soman-inhibition decreases fluctuations in some residues (e
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. Hydrodynamic calculations to predict molecular tumbling times were carried out on different models of the CaM:SEF2-1mp complex
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. The BLG solutions were prepared using the osmotic stress technique
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. This analysis can potentially allow us to discriminate between A-state monomers, protein oligomers, and larger aggregates
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. Histology and electron microscopy confirm these findings, indicating that pellets maintained in DMEM/ITS+/TGF-[beta]1 possessed an extensive extracellular matrix (Fig
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. It is obvious that this also can have different effects on the microenvironment of tryptophan residues
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. ThT dramatically increases its fluorescence quantum yield when bound to amyloid fibrils; however, the mechanism behind this property is unknown
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