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© 2007 by the Biophysical Society 0006-3495/07/01 /208/09 $2
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The first is a local length scale that spans seven-residue stretches
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From these findings it was deduced that the solidification of water affects the tertiary structure of azurin and it was suggested that binding of the fluorescence probe could represent a general approach to monitor ice-induced strains on the protein native fold
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Modem NMR Techniques for Chemistry Research
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Physical chemistry of protein solutions
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2 M) into a globule (Fig
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ANS, Bis-ANS, DCVJ, and ThT fluorescence as probes of different TTR conformersFluorescence spectra of the different molecular dyes in the presence of native and misfolded aggregated states of TTR are displayed in Fig
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The quantum yield of bound ANS is much higher than that for Bis-ANS, and the blue shift is more evident
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The unfolding was triggered at t = 0 by a temperature-jump from 25°C to 75-95°C and was monitored by CD at 222 nm (Fig
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2] of sea surface) were obtained in December 1986, 1996, and 1999, off the coast between Engafio Bay and Isla Escondida
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Each 8-ns trajectory was divided into 16, 8, and 4 blocks of non-overlapping trajectory segments of 0
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Our results illustrate how these secondary structure elements combine to direct the tertiary folding pathway of the ribozyme
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11At the clinical level, the distinction between reactive mesothelial proliferations and mesothelioma remains a major diagnostic problem
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Rao J, Otto WR
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"I'm just waiting until the time comes when I can afford the medicine I need," she says
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. The results of this study suggest that the ANS binding method may find practical utility in testing the effectiveness of various additives employed in protein formulations as well as to devise safer freeze-drying protocols of pharmaceutical proteins
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. ANS and DCVJ show significant fluorescence after l min
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