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. Since no crystal structure of ACYI is available, all four of the components (A and S, I, II, and III) of Trp fluorescence defined previously (33,34) were included in the curve-fitting process
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. CONCLUSIONS In summary, our work shows that the nonglycosylated form of SBA unfolds via a non-two-state pathway, in contrast to the glycosykued naturally occurring protein
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. and thus the molten-globule of EI^sup sc^ could have some of its dimeric interface partially disrupted
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. Collard; personal communication; March 2006), and indeed, it may play a role in their pathogenesis
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. The fluorescence of tryptophan residues is very sensitive to the local environment
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. Due to a rather slow structural unfolding but a fast irreversible step we observe an unfolding process which is under kinetic control
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. This is based on the observations that the bound SEF2-lmp lacks any well-defined conformation, and that not all hydrophobic peptide residues simultaneously interact with the hydrophobic patches on the inner surface of the CaM dimer (11)
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. Mutation studies on E12 (a bHLH protein similar to SEF2-1) have shown a gradual decrease in CaM affinity upon each mutation of an interacting residue, and not the on-off behavior usually seen with lock-and-key or induced-fit binding mechanisms (15)
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. The most pathogenic transthyretin variant, L55P, forms amyloid fibrils under acidic conditions and protofilaments under physiological conditions
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. It appears that insulin aggregation must proceed through a bulky, non-native monomer (Ahmad et al
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. The compact mature fibrillar structures formed at the endpoint of the aggregation process may hence result from an effort to minimize the exposure of hydrophobic residues
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. The use of solvent dye instead of the pigment may warrant investigation
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. FRET histograms were fit with multiple Gaussian functions using Origin (OriginLab Corp
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. Gβ^sub 1^γ^sub 2^, termed Gβγ, was expressed in StV cells using a baculovirus system and purified as previously described (see Runnels et al
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. Studies on insulin derivatives established that the two B-chain's termini might play distinct roles in the amyloidogenesis: the C-terminal slows down the process, whereas the residues at the N-terminal are necessary for the lateral aggregation, i
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