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On the basis of our results, we propose that assembly of the native GR dimer proceeds via initial formation of MG monomers that dimerize to form a partially folded, expanded dimer
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We could then discuss the relation between structure change and hydration of protein, together with other thermodynamic parameters
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The interactions between the attached glycans and the protein were assessed using the online LPC server (24)
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Octanol-Water Partition Coefficients: Fundamentals and Physical Chemistry
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This diffusion process continues through the channels of the IMC layer, resulting in an increase in the IMC layer thickness
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6 when the protein has a high net positive charge (dimer > +36e) are lower, indicating the presence of attractive forces and aggregation processes under these conditions
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Further maturation of the aggregates showed shrinkage of the spheres as the fibrils grew in length, suggesting a conformational change of the spheres into more rigid structures
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The average disk diameters were determined from EM images with an accuracy of 0
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This supports the proposed mechanism of the early fibrils shown in Fig
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Materials and methodsFish larvae and posttransition juveniles were collected during 19 research cruises conducted by INIDEP (Instituto Nacional de Investigaci6n y Desarrollo Pesquero) between 1978 and 2001
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0] m), and 34 and 79 m in fall surveys (mean depth [[+ or -]SE]: 60
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Small particles were suspended in the solvent using the particle size analyzer, then an extraction process [10] was run with acetone and methanol (Figure 3)
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To ensure that no eggs were lost after capture, these fish were immediately placed into plastic bags in order to retain any eggs that might be extruded before the ovary could be processed
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