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. Light scattering To estimate protein aggregation occurring upon thermal unfolding, the RF-1501 Fluorospectrometer was employed to measure elastic scattering at 500 nm
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. In contrast PPA shows already in the native state a rather red-shifted emission spectrum (λ^sub max^ = 343 nm) which first decreases in intensity upon heating
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. The effects of glycosylation, oligomeric state and differences in catalytic activity on molecular dynamics: AChE versus BChEGlycosylation appears to play a role in prolonging the circulatory lifetime of BChE and AChE (60,61), although it is not required for enzymatic activity
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. These observations are consistent with reports of the solidlike behavior of relatively dilute globular protein solutions (0
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. Unlike PH-PLCδ^sub 1^, the membrane binding face and orientation of PH-PLCβ^sub 2^ are unknown
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. Thus, the initial state in the first jump was intact disks, and in the second jump it was a mixture of protein-containing vesicles and lipid-poor protein
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. A thorough examination of these initial forms is of interest, as in this early timescale of the aggregation process both the nucleation and elongation coexist, and may influence each other
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. 95, respectively, based on the observed endpoints in the E shifts in Fig
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. Hydration study of proteins in solution by microwave dielectric analysis
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. Thus the sample with a concentration of 1 mg/ml was used for further analysis
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.5 M denaturant concentration also exibits a loss in the value of the mean residue ellipticity, suggesting that there is a loss in the secondary structure of the protein (Fig
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. However, our data indicate that aggregation and in addition at least one of these other mechanisms seems to play a role for the α-amylases with red shifts upon thermal unfolding (i
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. 5 B indicate that pressure does not promote the transition of PLCβ to an activated conformation
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. We define this reference time as the average burst duration within the 0
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