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Fifty minutes later, two Republicans succumbed, and after the longest, most unorthodox roll call in U
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For small x, the discrepancy is even more pronounced
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We have shown that there are two distinct length scales for proteins in the EV limit
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Reconciling sequence-specific conformational preferences with generic behavior of denatured proteins
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Those findings suggest that 1)
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The peak maxima range from ~308 nm for relatively nonpolar environments inside the protein globule to λ^sub max^-values of 350 nm which represent chromophores at the protein surface surrounded by polar water molecules (5,6)
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Doubly distilled milliQ water was used throughout
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In agreement with the previous report (12) for WT azurin, the intensity is enhanced ~7-fold and the spectrum is blue shifted by 13 nm relative to the ANS control
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Freeze denaturation of enzymes and its prevention with additives
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91 for aceturate, which agrees well with the respective values of 1
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In the following, this sample is denoted as DmAChE-H2O
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Percolation behavior of permeable and of adhesive spheres
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In contrast, addition of DCVJ and ThT to misfolded aggregates generated from A-state TTR results in a dramatic increase in the quantum yield of the different probes (Fig
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However, it can be a factor in finished ice cream where fat globules are intimately associated with oxygen at the fat globule/air cell interface
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Treating pellets as individual samples increases the catch to a pheasant, two robins, two cardinals, four rabbits, and three voles
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Factors affecting meal to pellet intervals in Great-horned Owls
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As a result, the
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Rocco sweeps in the chips, all of them the right ones
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The cockpit also features a digital moving map in which data can be preprogrammed in a "computer brick" in the squadron's planning spaces, carried to the aircraft and plugged in for a specifically tailored mission, a system similar to that in Hornets and Harriers
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. The thermal stability of immunoglobulin: unfolding and aggregation of a multi-domain protein
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. Thermal denaturation of rSBA and gSBA Thermal unfolding profiles of both nonglycosylated and glycosylated SBA were monitored by the change in molar ellipticity value at 218 nm at a protein concentration of 13 µM
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. Similar data were obtained for all protein sequences shown in Table 1
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. This simultaneous unfolding is strongly pH-dependent, and at high pH
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. In addition, other properties of the unfolded states, most probable structural compactness or simply differences in the conformational scrambling, also affect the magnitude of red shifts
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. The results of this study suggest that the ANS binding method may find practical utility in testing the effectiveness of various additives employed in protein formulations as well as to devise safer freeze-drying protocols of pharmaceutical proteins
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. Notwithstanding the relevance of the phenomenon, little is known of the structure of proteins in ice owing primarily to the inapplicability or the poor sensitivity of usual spectroscopic techniques in this highly scattering and anisotropic medium
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