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This investigation aims to complement that initial study by addressing the following issues: i), establish whether the perturbation of protein structure reported for azurin is general to other proteins; ii), inquire on potential correlations between the susceptibility to the ice-induced perturbation and specific features of the macromolecule such as thermodynamic stability, internal flexibility, internal cavities/hydration, and covalent links between strands; and finally iii), test for a specific vulnerability of multimeric proteins to freeze damage that may be linked to the disruption of quaternary structure
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Fluorescent labeling and measurementsProteins were labeled with 7-diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin (CPM)
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McLeish, University of Leeds, Physics and Astronomy, Woodhouse Lane, Leeds LS2 9JT, UK
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