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2 mg/ml) similar to that used for the activity assay, except that the transitions occurred at a temperature ~2°C higher than the 1 mg/ml sample
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In principle all these phenomena can alter the microenvironment of the intrinsic fluorophores and would therefore affect the emission spectra
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A change in the conformation of prions accompanies the emergence of a new prion strain
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5 and 6, and several corresponding pairs of rate constants (data not shown), and find these to be poorer descriptions of the experimental data as well
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