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Attributes of Glycosylation in the Establishment of the Unfolding Pathway of Soybean AgglutininSinha, SharmisthaABSTRACT Soybean agglutinin (gSBA) is a tetrameric legume lectin, each of whose subunits are glycosylated
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By and large, the hydrogen bonds range from 2
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To achieve subcellular nonwater ^sup 1^H MRS, the MR setup has to be highly sensitive, invoking the need for a strong static magnetic field, strong gradients, and micro radio frequency (rf) coils
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The evident binding of these four different dyes to misfolded conformations of TTR indicates their potential use as tools for following the misfolding and aggregation process
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DCVJ and ThT bound to aggregated TTR shows markedly similar fluorescence lifetimes which were overall short with at least three components (Fig
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EM images show that incubation of intact disks at high temperatures leads to disk fusion into small unilamellar vesicles, followed by fusion into large, possibly multilamellar, vesicles
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The unfolding was triggered at t = 0 by a temperature-jump from 25°C to 75-95°C and was monitored by CD at 222 nm (Fig
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Effect of denaturant and Chevron plotsAs central a tool to the experimental elucidation of folding kinetics as the protein engineering extraction of Φ-values is the tracking of folding rate with denaturant concentration
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Interpreting the folding kinetics of helical proteins
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A few experienced captains were able to target P
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A peak close to zero FRET efficiency is always observed, and occurs in part due to photobleaching of the acceptor, as well as other processes that give rise to donor-only molecules (Deniz et al
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These distances are in agreement with the corresponding values obtained from the present spFRET experiments (see Results section above)
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9 However, in some cases the implant failed and only fibrous tissue was produced
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Holtzer, Abbott J, Lash J, Holtzer S
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