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. 02 (Perkin-Elmer) with a γ factor of 2
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. Yet the probe sphere always intersects the chain unless it is centered sufficiently far away from the center of mass
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. 80, and τ^sup o^^sub m^ = 10 ns, an R^sub 1^ value of 1
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. A total of 25 µl 6 × SDS sample buffer (final SDS concentration of 2
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. Comparison with apoA-1 and apoC-1To assess the relative stability of the DMPC complexes with various human apolipoproteins, we used DSC, CD, and light scattering to monitor heat denaturation of similar-size DMPC disks with apoA-1, apoA-2, or apoC-1 under otherwise identical conditions
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. 4, 6, and 7) that promote protein dissociation, thereby creating a mismatch between the depleted particle surface and its core that leads to fusion
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. An interesting aspect of the late stages of the amyloidogenic assembly is provided by AFM data on insulin samples heated at 70°C for 20 (Fig
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. Perhaps the most remarkable example is the determination by fast kinetic experiments that β -lactoglobulin employs a transient helical motif that is entirely nonnative (Park et al
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. CD4-induced interaction of primary HIV-1 gp120 glycoproteins with the chemokine receptor CCR-5
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. The observation of well-resolved subpopulations in the FRET efficiency histogram indicates that the ribozyme can adopt two distinct conformations in solution
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. New insight into protein secondary structure from resolution-enhanced infrared spectra
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. However, at least on a qualitative level in particular differences between several unfolded states can be detected easily
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. A 45% decrease in activity was recorded after the neutron scattering experiment
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. In fact, the pattern of (τ^sup o^^sub m^)^sub app^ back calculated from the CaM:SMLCK complex when the diffusion tensor would be oriented along an axis perpendicular to the real diffusion tensor closely matches the pattern of (τ^sup o^^sub m^)^sub app^ found for the dimeric CaM:SEF2-1mp complex, Fig
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. 6 and Table 1) shows:i
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. Resolution of a signal transfer region from a general binding domain in Gβ for stimulation of phospholipase C-β2
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. In this study, however, we have also observed, along the typical transient structural motifs, that lateral interactions normally dominating at the later stages of fibrillogenesis and responsible for the thickening of the fibers may, in fact, play a role at the very beginning of the process-and lead to an alternative subpathway of amyloid assembly
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. The magnetite crystals were found to have a variety of shapes, to have linear dimensions of predominantly 10 to 100 nm (in the superparamagnetic-to-single-domain size range), to be chemically pure, and to be free of structural defects (see figure)
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. and plotted in the form of a histogram
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