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. The N [arrow right] I transition, which was referred to as the pretransitional change, ended at ~68°C and the T^sub 1/2^ occurred at ~60°C (see Table 1)
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. The position of attachment of each of the glycans ht a monomeric subunit is shown in Fig
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. We have carried out a systematic analysis to assess the quality of data obtained using the protocol described above
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. Thus, incubation of intact disks at 75°C for 30 min leads to disk fusion into enlarged particles that form stacks similar to disks but have larger diameters (d ≥ 25 nm) and occasionally form U-shaped bilayer structures typical of collapsed protein-containing vesicles (Fig
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