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Abbreviations used: PLC, mammalian phosphoinositide-specific phospholipase C; PI(4,5)P^sub 2^, phosphatidylinositol 4,5- bisphosphate; POPC, 1-palmitoyl-2-oleoylphosphatidylcholine; POPS, 1-palmitoyl-2-oleoylphosphatidylserine; PE, 1-palmitoyl-2-oleoylphosphatidylethanolamine; PH, pleckstrin homology; DNS-PE, dansyl phosphadtiylethanolamine; FRET, fluorescence resonance energy transfer instant decision unsecured loan .
To test whether the heating of apoA-2:DMPC disks to 100°C followed by incubation at 24°C (where protein-DMPC binding is fastest) leads to reconstitution of intact-size disks, the sample was incubated between the consecutive DSC scans at 24°C for up to 48 h instant decision unsecured loan .
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