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2]-glycoprotein-I, protein S, and annexin I
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Azodicarboxylic acid (diamide) was used to oxidize the cysteines to form the disulfide bridge and was present in all NMR samples
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ThT binds to oligomers and shows large amplitude in the slow second phase in response to formation of spherical aggregates and protofibrils, well in accordance with its reported amyloid specificity (Naiki et al
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After 48-h incubation, the disk reconstitution appears incomplete and a sharp lipid transition of reduced amplitude is detected (Fig
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The approach will additionally help us to see how the existing apparently-distinct paradigms for protein folding are related, and suggest places to look for the information content of the "kinetic code" within proteins that encodes the folding search path, as distinct from the native structure itself
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Consistent with this hypothesis, previous trFRET studies have shown that the 4WJ stabilizes the tertiary structure of the hairpin ribozyme by reducing the entropie penalty for docking as compared with ribozyme constructs containing either a 2WJ or 3WJ (Klostermeier and Millar, 2000)
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