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Lectin purification on affinity columns containing reductively aminated disaccharides
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that the large amphipathic surface in the dimer interface could be exposed to solvent upon monomer formation; furthermore, there is a network of aspartic residues involved in salt bridges at the dimeric interface (Fig
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Fast and one-step folding of closely and distant related homologous proteins of a four-helix bundle family
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As this is not available one can at best calculate the minimum average number of binding sites per macromolecule, n, by assuming the maximum fluorescence intensity increment, ~110-fold, ever observed between free and protein-bound ANS (φ^sub Fprotein^/φ^sub Fice^ = 0
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Extracellular-intracellular distribution of glucose and lactate in the rat brain assessed noninvasively by diffusion weighted 1H nuclear magnetic resonance spectroscopy in vivo
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, it weighs contributions from all atoms), it cannot be ruled out a priori that soman-inhibition decreases fluctuations in some residues (e
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Indeed, engineered HuBChE with a reduced degree of glycosylation displays catalytic properties similar to those of the wild-type enzyme (62)
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Collapse of single DNA molecule in poly(ethylene glycol) solutions
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0 no salt), and soluble misfolded oligomeric state (pH 2
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When comparing ThT and DCVJ fluorescence data from a protofibrillar sample of TTR (24 h incubation), it is evident that DCVJ shows pHindependent fluorescence (Fig
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High-resolution nuclear magnetic resonance studies of proteins
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In the same way, if denaturant concentration affects the stability of the low-dimensional search spaces, or gutters, that are principally responsible for controlling the folding rate, then these experiments will also carry signatures of the search-structure
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