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The ease managers get involved on the front end to review medical necessity and identify when a patient's insurer requires notification of an inpatient admission
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. Compared to native GR (circles), addition of 3 M GdnHCl (triangles) led to a significant increase in quenching efficiency, as indicated by an increase in K^sub SV^ from 12
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. Protein measurement with the folin phenol reagent
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. For small x, the discrepancy is even more pronounced
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. Beyond this length scale, all internal distances scale with sequence separation in accordance with the standard power law for proteins in a good solvent
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. (60) The therapeutic potential of these drugs has already been recognized, as is demonstrated by their use in patients with CF, asthma, and panbronchiolitis
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. Dissecting the vibrational entropy change on protein/ligand binding: burial of a water molecule in bovine pancreatic trypsin inhibitor
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. The interaction neither belongs to the category of lock-and-key nor to that of induced-fit
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. The data presented in Fig
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. Taken together, our results suggest that these hydrocarbon chain modifications do not play a significant role in the membrane binding stability of these proteins in accord with previous results (Philip and Scarlata, 2004)
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. The use of high pressure also allowed us to determine the volume change associated with the interaction between Gβγ and PLCβ
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. Interestingly, the midpoint and shape of the high-temperature DSC peak do not significantly change in the first and consecutive scans (curves 1-4 in Fig
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. This suggests that the once-formed fibroid enhances-through lateral interactions-formation of subsequent fibers
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. Immediately after collection, fish eggs were fixed in a solution of 5% formaldehyde to sea water solution and stored
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. For the hairpin ribozyme, the best fit to the donor decay was obtained using a model of two Gaussian donor-acceptor distance distributions, with mean distances of 34-37 [Angstrom] and 75-78 [Angstrom] (N
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. We did not observe any differences in the extent or intensity of immunostaining in cases in which a positive reaction for TGF-[alpha] or EGFR was encountered (Figure)
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. The PH domains of the enzymes are responsible for their different membrane binding characteristics; PLCδ^sub 1^ and its isolated PH domain binds specifically to membranes containing its substrate PI(4,5)P^sub 2^ through a series of specific hydrogen and ionic bonds (Ferguson et al
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. Our pressure studies show that the volume change for this association is twice as high in solution as on membranes
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. In the case of the SL 4WJ, for the broad/asymmetric peaks at some of the high Mg^sup 2+^ concentrations, these fitted Epeak values are representative of the peak positions, but are higher than the average FRET efficiencies by ~0
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. Give them a second copy of Reproducible 1
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