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States role the fishing in their waters up to three miles out
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For indole in aqueous solution, k^sub q^ for iodide quenching is 6
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Moreover, resolution-enhancing techniques, such as second derivative and 2D IR, have been shown to be sensitive tools to monitor the sequential events in protein conformational changes by FTIR (27,28,30,38-42)
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1-cm path length cuvette on a Jasco spectropolarimeter 715 (Jasco, Tokyo, Japan) attached to a Peltier PTC-348 WI
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Thermal denaturations following the ANS fluorescence did not show a native baseline, and a sigmoidal-like decreasing of the fluorescence was observed as the temperature was raised (data not shown)
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NaCl suprapur was purchased from Merck (Darmstadt, Germany) and ANS from Sigma (St
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In the liquid state the fluorescence of ANS is not affected by the addition of azurin, WT, and mutants
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Proton chemical shifts were calibrated using the internal standard DSS (0
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In general, the application of hydrostatic pressure in the range of 1-2000 bar (where 1 bar = 1
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1 B and by their independence of the scan rate), the heating and cooling curves of the apoA-2:DMPC complexes show hysteresis that was also observed in DMPC complexes with apoC-1 (Gursky et al
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Although the time of the disk reconstitution may vary for apoA-2 from different plasma pools, the general trend is similar and shows a relatively slow vesicle-to-disk conversion
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One of these pellets (catalogue USNM 601957) is unusual because it contains a large (> 190 mm long when extended), complete primary (P-9) feather (Fig
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Two factors may account for the lack of bird bones in these pellets
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9 billion in drug expenditures between 2006 and 2015--this includes $44
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