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5 mM PEP and 5 mM Mg^sup 2+^), and every digestion was repeated three times
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Two protease inhibitor homologues from black mamba venom
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In the case of circular dichroism (CD) spectroscopy, the same buffers was used, but with 10 mM Mops
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2-cm cell at a protein concentration of 0
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ANS Fluorescence Detects Widespread Perturbations of Protein Tertiary Structure in IceGabellieri, EdiABSTRACT Freeze-induced perturbations of the protein native fold are poorly understood owing to the difficulty of monitoring their structure in ice
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Dynamics of ANS binding to tuna apomyoglobin measured with fluorescence correlation spectroscopy
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Other MD studies have demonstrated that transient openings in the wall of the gorge, so-called back-doors or side-doors, provide putative alternate routes for traffic of substrate, products, and/or solvent (29,30)
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Identification of nanosecond-timescale internal motionThe presence of nanosecond-timescale motion can be directly identified from a plot of the normalized ratio of R^sub 1^ values measured at two magnetic fields (RT^sup n^^sub 1^) versus NOE (22)
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The discrepancy at the lower concentrations (mass fraction ~7-16% w/w) could be decreased if the assumed molecular weight (MW) were reduced from the dimer MW to the monomer MW
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Our laboratory has previously characterized the affinity between PLCβs and their G-protein activators on model membrane surfaces using FRET (Runnels and Scarlata, 1999)
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An advantage of this PLCβ construct is that it can be bacterially expressed (see Wang et al
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The fibers are considerably thicker (the average diameter judged from the height data is 9
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The observed tendency of untwisted lateral agglomerates (Fig
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