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. MATERIALS AND METHODSMaterialsGR (type IV, from Baker's yeast), GSSG, NADPH, DTNB, GdnHCl, and HEPES were from Sigma Chemical (St
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. The changes of thermodynamic parameters (enthalpy change ((Delta)H), entropy change ((Delta)S) and heat capacity change ((delta)C^sub p^)) corresponding to the transitions from N to MG (molten globule state) or MG to U (unfolded state), were summarized in the previous report (Nishii et al
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. The proteins were redissolved in D^sub 2^O and centrifuged at 6000 × g for 10 min before use with a final pD of 8
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. In Protein Folding Handbook, Vol
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. Analysis of pH-dependent folding and stability of histidine point mutants allows characterization of the denatured slate and transition state for protein folding
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. In the vegetal cytoplasm, the aceturate peaks are still smaller than in the animal cytoplasm, concordant with the vegetal cytoplasm being even more stuffed with maternal macromolecules (31,39,40)
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- Silver Street Development Corporation vice president Drew Fitch joined Arbor Commercial Mortgage and Fannie Mae in planting trees as they recently celebrated Arbor Day and the grand reopening of the New Brunswick Apartment Complex in New Jersey[ILLUSTRATION OMITTED]Caption: Silver Street Development Corporation vice president Drew Fitch (pictured left) joined Arbor Commercial Mortgage and Fannie Mae in planting trees as they recently celebrated Arbor Day and the grand reopening of the New Brunswick Apartment Complex in New Jersey
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. The output signal was digitized and stored by a multi-function board (PCI-20428W, Intelligent Instrumentation, Tucson, AZ) utilizing visual Designer software (PCI-20901S version 3
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. Effects of cavity-forming mutations on the internal dynamics of Azurin
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.HuBChE-D^sub 2^O versus Soman/HuBChE-D^sub 2^OMSDs for the native and the inhibited enzyme are identical in the temperature range below 334 K (61°C) and increase smoothly (Fig
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