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The helix-loop-helix motif formed by the α-helix 9-α-helix 10 shows intermonomer salt bridges: Asp^sup 540^ in the motif with Arg^sup 422^' in α-helix 5 from the other monomer (the prime is used to designate residues in the other monomer) large loan unsecured .
However, we cannot rule out that an alternative explanation to the titration curve is that the deprotonation of a histidine residue can be felt by a residue(s) far enough in the three-dimensional structure (see Fersht (45) and references therein) affecting its (their) environmcnt(s) large loan unsecured .
Here, the much greater intensity enhancement observed for the mutants, compared to the WT, stands to indicate that the number of ANS binding sites and, consequently, the magnitude of the structural alteration are sensibly larger with these less stable azurins large loan unsecured .
laevis oocytes fit this condition large loan unsecured .
Figure 8 exhibits the dependency of wetting force and meniscus height on the perimeter (P) of the substrate large loan unsecured .
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Here, we have used fluorescence methods to follow the membrane association of a chimeric PLCβ/δ, referred to here simply as PLCβ (see Methods) under pressure large loan unsecured .
The importance of diffusive searches is unsurprising, since under biological conditions, all candidates for energetic interactions, including electrostatics, are locally screened to a few angstroms: much smaller than the dimensions over which sections of protein must move to find their native configurations large loan unsecured .
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Ware JE, Kosinski M, Keller SD large loan unsecured .
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