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. The helix-loop-helix motif formed by the α-helix 9-α-helix 10 shows intermonomer salt bridges: Asp^sup 540^ in the motif with Arg^sup 422^' in α-helix 5 from the other monomer (the prime is used to designate residues in the other monomer)
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. However, we cannot rule out that an alternative explanation to the titration curve is that the deprotonation of a histidine residue can be felt by a residue(s) far enough in the three-dimensional structure (see Fersht (45) and references therein) affecting its (their) environmcnt(s)
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. Here, the much greater intensity enhancement observed for the mutants, compared to the WT, stands to indicate that the number of ANS binding sites and, consequently, the magnitude of the structural alteration are sensibly larger with these less stable azurins
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. Here, we have used fluorescence methods to follow the membrane association of a chimeric PLCβ/δ, referred to here simply as PLCβ (see Methods) under pressure
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. The average shape of a denatured protein is that of a prolate ellipsoid We computed the ensemble-averaged asphericity values for each of the 23 sequences in the EV limit and the resultant data are shown as cross marks in Fig
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