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. In a previous study, we probed the role of the two proximal GlcNAc residues in tethering the glycans attached to one subunit to the side chains of amino acid residues of an adjacent subunit at the noncanonical interface (18)
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. It is apparent that the role of glycosylation in maintaining conformational stability is more pronounced compared to regulating the ultimate functional form of the protein
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. uk) by using the Needleman-Wunsch global alignment algorithm (with the following parameters: matrix: EBLOSUM62, gap penally value: 10
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. This feature can either by related to arising tyrosine emission (often observed around 305 nm for unfolded states when energy transfer to tryptophans disappears during a structural expansion of the protein; see, for example, Schmid (20)) or to elastic scattering caused by aggregation
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. Stimulated by the biological importance of this subject, many in vitro investigations of DNA interaction with K+ and Na+ ions as well as other monovalent alkali cations (Li+, Rb+, and Cs+) have been performed by biophysicists (Bleam et al
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. ThT does not show binding to the U-state and only shows a minor burst phase, indicating that this is a clearcut aggregation probe
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