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The least well known of this set is, musically speaking, no great shucks
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. The 2D IR plots were presented as contour maps produced by drawing the contour lines every 10% off from the maximum intensity of the whole correlation map
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. The histidine-phosphocarrier protein of Slreptomyces coelicolor folds by a partially folded species at low pH
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. In these exploratory experiments conducted with copper-free azurin from Pseudomonas aeruginosa as a model protein system, it was shown that upon freezing of the protein solution the fluorescence of ANS is enhanced severalfold and becomes blue shifted relative to free ANS
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. 1 M potassium cyanide and 1 mM EDTA in 0
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. Thanks to an unusually blue shifted Trp fluorescence spectrum of native azurin, a measure of the protein fraction remaining native after freezing-thawing can be obtained directly from the residual fluorescence intensity at 300 nm (λ^sub ex^ = 292 nm), a wavelength where denatured-aggregated proteins were found to contribute little to the overall intensity
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. , (48)), thus compensating for the reduced flexibility adjacent to the binding site
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. Unlike PH-PLCδ^sub 1^, the membrane binding face and orientation of PH-PLCβ^sub 2^ are unknown
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. Evidence for a unique folding intermediate in the 4WJ ribozymeOur results indicate that the SL 4WJ ribozyme can fold into a compact conformation in the presence of high Mg^sup 2+^ concentrations, with arms A and B juxtaposed in a similar fashion to the docked native 4WJ ribozyme
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.HPLC analysisGR samples (5
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