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. Molten globule monomers have been proposed as intermediates in the dissociation of some dimeric proteins, including human superoxide dismutase (Silva et al
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. The protein was eventually cluted in 0
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. The excess heat capacity functions were then analyzed using the software package Origin 7
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. MATERIALS AND METHODSEnzyme purification and characterizationHuBChE was purified to homogeneity from outdated human plasma according to an improved protocol based on that of Grunwald et al
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. This is a common be havior of protein phase diagrams and of particles with shortranged attractive interparticle forces, e
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. According to the polymeric paradigm (Fändrich and Dobson, 2002) of amyloidogenesis and the therein-implicated importance of backbone interactions, the more the native structure becomes distorted (and the protein backbone becomes exposed), the higher the chance that the protein steps onto the amyloidogenic pathway
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. 1 and then normalized by dividing it by the number of atoms of each residue
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. Thermal denaturations following the ANS fluorescence did not show a native baseline, and a sigmoidal-like decreasing of the fluorescence was observed as the temperature was raised (data not shown)
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. It was compared to a native HuBChE sample, denoted HuBChE-H2O (0
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