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We compare the costs involved in similar surgical cases
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. Interestingly, the sample with removed GndHCl exhibits now a strong elastic scattering related to pronounced aggregation, which was not observed under unfolding conditions with 8 M GndHCl
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. Also plotted in Fig
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. It appears that insulin aggregation must proceed through a bulky, non-native monomer (Ahmad et al
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. These distances are in agreement with the corresponding values obtained from the present spFRET experiments (see Results section above)
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. In those 2 studies, however, the staining was entirely cytoplasmic, whereas the staining observed in our cases was mainly located on the cell membrane, with only some marginal cytoplasmic immunoreactivity
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