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Apparently, glycosylation promotes the protein with greater stability, which is considerable in its magnitude (9 kcal/mol)
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The stability of the monomer of rSBA is comparable to many monomeric proteins of similar size
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However, at the relatively high CaM concentration (1 mM) used in the NMR experiments, compared to the nanomolar dissociation constant of the complex, all CaM is in the bound state
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Time-resolved dynamic light scattering
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The phenomenon of single molecular DNA compaction is expected to lead to higher order DNA structures with a high degree of packing of DNA segments, however the volume of DNA condensate can be also dependent on the nature of monocation
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Before initiation of the aggregation reaction, TTR is in the form of unfolded monomers
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, 1997; Sipe and Cohen, 2000; Jackson and Clarke, 2000; Serpell, 2000; Dobson, 2003; Rochet et al
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The solvent dye is well distributed throughout the particles
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- Fannie Mae also lobbied Congress to cut OFHEO's funds unless it got rid of the top official in charge of investigating Fannie Mae
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. ACYI contains eight Trp residues in each of its two subunits (47,48), and the eight Trp residues are distributed in both the peptidase and dimerization domain (13,17)
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. This different behavior in the thermograms among the members of a protein family is not new, and it has been reported, for instance, in the thermodynamic unfolding of dimeric histone-like proteins (53)
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. This approach has been employed here to deconvolute the fibrillar structure shown in Fig
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. This is usually interpreted to mean that the residue in question enjoys the majority of its native contacts at the transition state (Fersht, 2000)
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. In other words, the second MD trajectory segment covers a small subspace of the conformational space sampled by the first segment and hence reduces the overall size of the elements of the covariance matrix σ through averaging over the combined trajectories
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