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. However, it has been shown in various cases that (and how) under specific conditions λ^sub max^-signals can be used for quantitative analyses of unfolding transitions (4,12,15)
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. In the liquid state the fluorescence of ANS is not affected by the addition of azurin, WT, and mutants
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.The widely used amyloid probe ThT appears to function with a similar mechanism, but in addition the local hydrophobicity has to be changed for the characteristic fluorescence of ThT to appear
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. Electron microscopy, gel electrophoresis, and differential scanning calorimetry show that such fusion involves lipid vesicle formation and dissociation of monomolecular lipid-poor protein
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.0 (0/5)AcknowledgmentsWe would like to thank the following sea farming centers and universities for providing the fish eggs used in this study: Fukui Prefectural Sea Farming Center; Kyoto Prefectural Sea Farming Center; Faculty of Agriculture, Kyushu University; Osaka Prefectural Fisheries Station; Sea Farming Center of the Japan Sea-Farming Association; Fisheries Laboratory of Kinki University
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