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In native proteins containing both tyrosine and tryptophan residues, tyrosine emission is not commonly observed due to efficient energy transfer from tyrosine to neighboring tryptophan residues (Lakowicz, 1983)
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In contrast to high temperatures the presence of GndHCl efficiently suppresses aggregation
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To contribute to the ongoing discussion and to investigate the relationship among molecular dynamics, activity, inhibitor-binding, and unfolding, we carried out incoherent elastic neutron scattering experiments on two members of the cholinesterase (ChE) family: Tetrameric human butyrylcholinesterase (HuBChE), both native and covalently inhibited by the organophosphate nerve agent, soman (18), and dimeric native Drosophila melanogaster acetylcholinesterase (DmAChE)
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.4 µM) were incubated for 90 min in the absence or in the presence of different GdnHCl concentrations and applied onto a Superdex 200 HR column (Pharmacia, Uppsala, Sweden), previously equilibrated with 20 mM Tris-HCl, at pH 7
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. The backbone (N, Cα, and C') atoms of individual residues were used for superposition of trajectory configurations when calculating the entropy per residue (code: fir)
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