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. For example, a sample that was used to record curve 1 in Fig
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. However, the herein-observed morphological variation after increasing temperature bears a more profound meaning in light of the ongoing debate as to the identity of the protein's aggregation-prone conformation (completely unfolded, or partly unfolded protein) and our own study, showing that under similar conditions, bovine insulin is substantially more unfolded at 70 than at 60°C (Dzwolak et al, 2003)
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. Karplus and Weaver (1994), for example, shows a fast folding route of hen lysozyme in which the early formation of β-sheet structure permits the final approach to the native state to proceed in a subspace of reduced dimension
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