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. All other reagents were local products of analytical grade
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. From 30°C to 50°C, no significant changes were observed tor the four components
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. The nonglycosylated form features a lower stability when compared to the glycosylated form
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. Protein concentration was calculated by using the extinction coefficients of Trp, Tyr, Cys, and Phe residues (24)
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. pH-induced structural changes Steady-state intrinsic fluorescence measurements
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. Control experiments were earned out at 7 M GdmHCl, pH 7, where the protein was fully unfolded
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. The deconvoluted bands in the FTIR spectra suggested that, within the experimental uncertainty, the percentages of secondary structure remained unaltered upon binding of both effectors (Table 4)
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. making the monomermonomer interaction less stable
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. Ca^sup 2+^ signaling can inhibit the transcriptional activities in vivo of the bHLH proteins E12 and SEF2-1 through direct binding of Ca^sup 2+^/CaM to the basic sequence of the proteins, resulting in inhibition of their DNA binding (15)
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. Further support for this hypothesis comes from a large reduction in the apparent T^sub m^ and faster thermal unfolding observed upon disulfide reduction in apoA-2:DMPC disks (data not shown), which is consistent with the report that apoA-2 on the disks is less stable in monomeric than in dimeric form (Lund-Katz et al
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. , 2003), which are likely to consist of tubularly-stacked infinite β-helices (Jimenez et al
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. The diffusing particle (representing of course the random search of the protein through its available conformations) does not have to search simultaneously through both the dimensions of the figure
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. BD/BLx100HD/BLx100Preflexion 3
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. , 2002), the conformational rearrangement in the bridging sheet of gp120 upon CD4 binding is accompanied by a significant loss of entropy
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. SuborderSpecies DistributionPagrinaePagrus major South of Hokkaido (Coastal)Evynnis japponicaSouth of Hokkaido (Coastal)SparinaeAcanthopagrus schlegeliSouth of Hokkaido (Coastal)Acanthopagrus latusSouth Japan (Coastal)Sparus sarba South Japan (Coastal)DenticinaeDentex tumifrons South Japan (Oceanic)Spawning Egg oil globuleSuborder seasonEgg size (mm)size (mm)PagrinaeMar-May0
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