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. The optimal temperature for ACYI activity is ~60°C (25,26), which indicates that it can be regarded as a thermostable enzyme
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. Theory predicts that ensemble-averaged internal distances will scale like ensemble-averaged end-to-end distances such that [left angle bracket]R^sup 2^^sub ij^[right angle bracket]/([left angle bracket]R^sup 2^^sub e^[right angle bracket]|i - j|) = [left angle bracket](r^sub i^ - r^sub j^)^sup 2^[right angle bracket]/([left angle bracket]R^sup 2^^sub e^[right angle bracket]|i - j|)~1 (39)
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. Excitation wavelength was 370 nm, and the emission intensity was collected from 430 to 700 nm
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. The solvent-accessibility of tryptophan and tyrosine residues was examined by iodide and acrylamide quenching at several pHs
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. The two cytoplasmic regions differed in their lipid contents and NMR lineshape characteristics-differences that are not detectable with whole-cell NMR techniques
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. The relaxation data were measured at multiple magnetic fields, and analyzed in a model-free manner using in-house written software designed to detect nanosecond internal motion
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. A relaxation-compensated Carr-Purcell-Meiboom-Gill sequence for characterizing chemical exchange by NMR spectroscopy
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. CPM-labeled proteins were excited at 385 nm and scanned from 420 to 540 nm
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. © 2005 by the Biophysical Society0006-3495/05/01/15/10 $2
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. This suggested that CG4 might display an unusual array of colors
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. They were fixed with 5% formaldehyde in sea water, either immediately or after incubation in seawater in finger bowls at 20[degrees]C for 24 hours, and identified by careful observation as described by Ikeda and Mito (1988) and Ikeda et al
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. We refer to this species as a quasi-docked state to denote the native-like arrangement of arms A and B, although there are no actual docking interactions between these arms because loop A is absent
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. J Immunol 2001; 166:6847-6854(35) Cowan KN, Leung WC, Mar C, et al
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. The scattered signals were corrected for container scattering, absorption, and instrumental resolution (determined by separate runs of the empty container and of a vanadium sample) by a standard FORTRAN 77 program Iq0 based on the correction formula of Paalman-Pings coefficients (42)
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. Increased protein backbone conformational entropy upon hydrophobic ligand binding
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.3-nm in diameter, with elongated features (Khurana et al
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.In contrast to the 60°C incubation, in which a fibril concentration increase of ~1:4 (235 fibers versus 325 fibers) can be determined in the time period between 1 day and 1 week, a noteworthy fibril concentration change at 70°C in the same period does not take place
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.To see how this works, we suppose at first that the d ~ O(10^sup 2^) dimensions of the full folding space are now arranged sequentially so that diffusive searches in one dimension at a time allow the protein to find "gateways" into the next subspace (we will see how this may arise naturally in a physical way below)
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.Configurational entropy analysis of appended or combined trajectories can provide insight into the relative size and overlap of the conformational space sampled by a pair of single trajectories
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