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. However, the molar ellipticity at 222 nm at basic pH was larger (in absolute value) than that at pH 7
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. Either we could not unambiguously determine the protein-concentration independence of the thermal midpoint from fluorescence measurements (see above, for the values, and Fig
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. Opposing effects of phosphoenolpyrm ate and pyruvate with Mg^sup 2+^ on the conformational stability and dimerization of phosphotransferase enzyine I from Excherichia coli
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. Compact intermediate slates in protein folding
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. When the binding reaches saturation, nonbound molecules dominate the diminazene signal in the nucleus
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. The reversible transformation of β-lactoglobulin at pH 7
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. Karplus and Weaver (1994), for example, shows a fast folding route of hen lysozyme in which the early formation of β-sheet structure permits the final approach to the native state to proceed in a subspace of reduced dimension
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. PFROMMDepartment of Chemical Engineering, Kansas State University, Manhattan, Kansas 66506, U
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. (8-10) The head or neck is involved in one third of cases
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. AcknowledgmentsThis study resulted from cooperative efforts of the USGS Biological Survey Unit and the Department of Zoology, National Museum of Natural History
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. 16 Re-expression of a differentiated phenotype is enhanced by the presence of defined growth factors, such as TGF-[beta], insulin or IGF-I
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. The Web site was developed from materials available from the Centers for Medicare and Medicaid Services and includes input from a multidisciplinary Advisory Panel, whose members include the American College of Physicians, the Access to Benefits Coalition, the American Medical Group Association, the National Family Caregivers Association, the Midwest Business Group on Health and the American Phamacists Association
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. More importantly, the position of the negative peak, which reflects the unfolding of the native structures, was centered at around 1628 cm^sup -1^ in the temperature range 30-66°C, then shifted to around 1639 cm^sup -1^ when the temperature increased from 66°C to 80°C, and finally remained at this wavenumber upon heating
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. The curves from the diluted solution (data not shown) were similar to those presented in Figs
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. The S^sup 2^^sub f^ and τ^sub if^ values extracted from M2II are presented in Fig
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.SUPPLEMENTARY MATERIALAn online supplement to this article can be found by visiting BJ Online at http://www
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. Millisecond-timescale motions contribute to the function of the bacterial response regulator protein Spo0F
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. 2-4, the osmotic pressure determined using the osmotic stress technique is plotted as the dimensionless compressibility factor, Π/ρk^sub B^T, as a function of protein mass fraction for pH 8
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. Evidence for sequential barriers and obligatory intermediates in apparent two-state protein folding
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