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But the complexity and sophistication of the efforts that have been needed to reach this resolution show how, at times, a simple question can open up penetrating new leads into the labyrinth of evolutionary theory
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Similarly, the free energies of unfolding of C^sub H^2 antibody domain and pliage P22 coal proteins are, respectively, 3
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In other words, although sequence specificity is not apparent in the average denatured-state topology, it is apparent in the way the symmetry characteristic of the denatured state is broken
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Antimicrob Agents Chemother 2003; 47:48-53(61) Rubin BK, Henke MO
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Further and more detailed insights in the mechanisms of how aggregation affects properties of the unfolded state can be gained from studies where aggregation, in particular of thermal unfolded states, is suppressed
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Being aware that the rotational correlation time of the aggregates were larger than the fluorescence lifetime of bound ANS, we generated a distribution of aggregate sizes using a variety of TTR concentrations from 2 to 8 µM (monomer concentration) that were incubated for 20 h (Fig
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Abbreviations used: PLC, mammalian phosphoinositide-specific phospholipase C; PI(4,5)P^sub 2^, phosphatidylinositol 4,5- bisphosphate; POPC, 1-palmitoyl-2-oleoylphosphatidylcholine; POPS, 1-palmitoyl-2-oleoylphosphatidylserine; PE, 1-palmitoyl-2-oleoylphosphatidylethanolamine; PH, pleckstrin homology; DNS-PE, dansyl phosphadtiylethanolamine; FRET, fluorescence resonance energy transfer
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, 2003), yeast prion protein Ure2 (Jiang et al
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In this case the mutual diffusion of the helical and β-sheet portions of the protein is the dynamical process responsible for the gutter-like feature on the reduced folding surface
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