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8 b shows the distribution of the iwo types of interactions
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For ubiquitin, we computed P^sub oa^(r) for probe spheres of radii ranging from 2
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An isolated three-stranded antiparallel β-sheet domain that unfolds and refolds reversibly: Evidence for a structured hydrophobic cluster in urea and GdnHC1 and a disordered thermal unfolded state
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2), we were able to estimate the self-association constant
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Therefore, the maximum of the fluorescence emission spectrum (λ^sub max^) is sensitive for the polarity of the microenvironment of the intrinsic fluorophore
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As a consequence the observed emission spectra of individual proteins (and of their corresponding conformational states) do not even vary with respect to λ^sub max^ but also can show different spectral shapes and widths (7,8)
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An estimate of the number of binding sites requires knowledge of the average fluorescence quantum yield (φ^sub F^) of ANS bound to azurin
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Note, however, that NMR relaxation data on various protein:target complexes do show a redistribution of motions upon specific target binding
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At small strains, γG' over the frequency range examined of 0
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As shown in Fig
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Based on the temperature determined by a probe, an automatic compensation was made to the position of the feature machined
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Since the docked and undocked peaks are well resolved, any interconversion between the two conformers must occur more slowly than the ~280-µs average burst duration of the single-molecule measurements (see below)
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Journal of Raptor Research 36:58-65
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Part II presents the positive critique, namely by setting out some proven and promising methodological alternatives to vivisection such as, among others, the epidemiological method, computer simulation and in vitro techniques
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These results are consistent with those obtained in a study conducted by Damush et al
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. The fluorescence emission of native GR is quite blue-shifted ([lambda]^sub max^ at 317 nm), indicating that, on average, the four tryptophan residues in each GR subunit are well-protected from exposure to the aqueous medium in the folded protein
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