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. At basic pHs, the K^sub sv^s were slightly larger than at neutral pH, but smaller than those at acidic pHs
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. In addition, other properties of the unfolded states, most probable structural compactness or simply differences in the conformational scrambling, also affect the magnitude of red shifts
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. For the hairpin ribozyme, the best fit to the donor decay was obtained using a model of two Gaussian donor-acceptor distance distributions, with mean distances of 34-37 [Angstrom] and 75-78 [Angstrom] (N
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. The energy difference ΔU = U - U' is evaluated
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. Thermodynamics of protein denaturution
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. The fact that the CD thermal transitions of EI^sup sc^ became broad as the concentration was increased suggests that the thermal transition of the EIC^sup sc^ is also being observed, as happens in EIec (52)
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. Three probes-ANS, DCVJ, and ThT-show increased fluorescence throughout the aggregation reaction, whereas Bis-ANS shows fluorescence decay (Fig
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.Address reprint requests to Per Hammarstrom
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. We have previously shown that Gα(GDP)-subunits cause de-activation of PLCβ-Gβγ without significantly affecting their association (Runnels and Scarlata, 1998)
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. The case of diffusion in mutual angular space of helical bundles discussed above is an example, since this occurs within a globule, rather than in the collisional formation of a globule
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