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. When excited at 280 nm, gSBA shows a fluorescence emission maximum at 328 nm, which is red-shifted to 354 nm on denaturation with 7
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. Recently, Zhou (114) computed the functional form of x^sup 2^P(x) from EV simulations of proteins studied by Wilkins et al
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. 2 should not be considered a binding curve, it may still be instructive to estimate an apparent average affinity of ANS for the four azurin species
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. No significant difference in the circular dichroism spectrum was seen in either the far UV (190-230 nm) or the near UV (260-300 nm) before and after the neutron scattering measurement
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. The simulation results were found very close to the experimental results
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. However, the present data cannot exclude internal motion involving reorientation of the CaM monomers, which have an excepted timescale of ~7-9 ns
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. Similar increase in fluorescence quantum yield was evident in 50% w/w PEG (MW 3350; data not shown) as in 50% glycerol showing that increased viscosity is the main reason for the increased quantum yield
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. Upon maturation the oligomers clustered into either protofibrils or spherical aggregates, and upon further maturation the spheres shrink in size as fibrils extend in length
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. 19Table 2Fish eggs used in the experimentEggStage of Samplingno
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. All SL constructs show just a single peak in the FRET efficiency histograms, with no distinct docked peak, as expected (Fig
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. We also discuss the significant destructive potential of BB and pellet guns
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. Canadian Journal of Zoology 60:1282-1288
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. 24The repair of chondral defects using ACI techniques requires the proliferation of cells and the elaboration of a GAG-rich extracellular matrix
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. ALL THIS SPOT TALK GOT ME WONDERING ABOUT ROGER SPOHN, THE SAD SACK MEDICAL MARIJUANA PATIENT 1 PROFILED IN A PREVIOUS STORY, * SO I MET UP WITH HIM AGAIN TO CATCH UP
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. Kratky profiles for proteins in the EV limit were compared to those of folded proteins and ideal, freely rotating chains (43)
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. ** SOGS score [greater than or equal to] 5
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