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Since K^sub SV^ equals the product of the bimolecular quenching rate constant (k^sub q^) and the excited-state lifetime of the fluorophore ([tau]), the value of K^sub SV^ obtained from steady-state fluorescence measurements is dependent on the average lifetime
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Taken together, these results suggest that at least two intermediate states can be identified in the equilibrium unfolding of GR, namely a partially unfolded predissociated state and a molten globule monomer
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The phase diagram analysis, which is a sensitive tool to detect folding intermediates (35), was performed to characterize whether an intermediate was involved in ACYI thermal unfolding
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This shoulder seems to represent a subpopulation of thermal unfolded states which exhibit less buried tryptophan residues, possibly related to unfolded proteins which are not aggregated
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Besides the shift of transition temperatures caused by different heating rates, pronounced differences in the λ^sub max^-values for the unfolded states are visible in Fig
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Thus, the solidification of water alters the structure of BSA, creating a looser fold able to bind numerous ANS molecules in its interior that exhibit large fluorescence yields
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In contrast to reports on DNA binding with monocations, the compaction potentials of monocations determined here correlate well with biological assays that describe the compaction of chromosomes
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Summary of the use of ANS, Bis-ANS, DCVJ, and ThT as fluorescence conformational probesThe results of the analysis of the fluorescence response in binding to unfolded monomers, native TTR, and various stages of the aggregation reaction is summarized in Table 2
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Large unilamellar vesicles (LUVs) were freshly prepared by extrusion through a 100-nm pore filter before every experiment
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1-fold activation of 2 nM PLCβ by 50 nM Gβγ (S
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In the same way, if denaturant concentration affects the stability of the low-dimensional search spaces, or gutters, that are principally responsible for controlling the folding rate, then these experiments will also carry signatures of the search-structure
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An example is given in Fig
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Glass transition temperature (T^sub g^) and melting temperature (T^sub m^) were determined by DSC (Seiko SII DSC 200C, Perkin Elmer Pyris I DSC)
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Small particles were suspended in the solvent using the particle size analyzer, then an extraction process [10] was run with acetone and methanol (Figure 3)
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However, ages have been validated for yellowtail rockfish (Sebastes flavidus), a closely related species (Leaman and Nagtegaal, 1987), by using otoliths; moreover, the break-and-burn aging method is widely accepted as valid for aging rockfish (MacLellan, 1997), and ages thus derived are routinely used in rockfish stock assessments
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ANCOVA also showed no significant difference in elevations (P=0
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There was no technical problem assaying 43 or 102 eggs from natural waters
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To better understand the basis for the observed broadening and tailing, we carried out numerical simulations using a simple model of the single-molecule FRET data
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Address reprint requests to Ashok A
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This version packs an extra punch of moisture
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On the basis of information from drug supply stakeholders, the FDA had expected this technology to be in widespread use in the drug supply chain by 2007
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For instance, regarding regulatory accountability, at what point is pharmacy practiced? At the point of dispensing a medication, in this case in Canada, or at the point of medicine delivery, which would be in the United States?One benefit of the communique may be to drive debate in Canada
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"The new pharmacy design eliminates all the glass, all the barriers that the pharmacist and the consumer encounter
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The franchisor's growth over the past three years is shown by the number of both franchise and company-owned units for 2002, 2003 and 2004
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