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. A figure presenting the matrix-assisted laser desorption ionization mass spectra of the glycosylated and nonglycosylated form is shown in the Supplementary Material
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. The shape of emission spectra as obtained from GndHCl-induced and thermal unfolded states also reveal some clear differences (Fig
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. We have developed a method for analyzing ^sup 15^N-spin relaxation measured at two magnetic fields that corrects (τ^sup B^^sub m^)^sub app^ for internal motion up to ~4 ns, giving (τ^sup o^^sub m^)^sub app-corr^ (22)
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. In Monographs on the Physics and Chemistry of Materials
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. The case of diffusion in mutual angular space of helical bundles discussed above is an example, since this occurs within a globule, rather than in the collisional formation of a globule
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. For the activity assays (see below), the substrate strand sequences used are 5'-GGC GUG GUA CAU UAC CUG GUA CCC CCU CGC AGU CCU A-3' (S2WJ), 5'-GUC AAC UCG UUC GCA GUC CUA-3' (S3WJ) and 5'-GCA AGC CAC CUC GCA GUC CUA-3' (S4WJ)
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. Lastly, kinetic information about equilibrium interconversion of subpopulations (on microsecond to millisecond timescales) is uniquely available from spFRET data
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. These results indicate a multistate folding pathway for GR and suggest a critical role of molten globule monomers in the correct dimerization of GR
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. Consideration of both typical range of stabilizing interactions, and folding rates themselves, leads to a model of the energy hypersurface that is characterized by the structure of diffusive "hypergutters" as well as the familiar "funnels"
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