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A further increase in quenching efficiency was observed at 7 M GdnHCl (squares, K^sub SV^ = 40 M^sup -1^), indicating that even at 3 M GdnHCl the tryptophan residues of GR were still significantly protected from iodide quenching relative to the fully unfolded protein
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In experiments with the effectors (5 mM Mg^sup 2+^ and 2
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1 M^sup -1^) similar to those measured in isolated EI^sup SC^ (Table 1), and smaller when PEP (1
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Over 100 years ago, Metchnikoff recognized the importance of the homeostatic removal of dead cells, which he termed physiologic inflammation
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The lowest protein concentration of 7 µg/ml reveals a large red shift with λ^sub max^ of ~345 nm, whereas protein concentrations of 50 µg/ml and 1 mg/ml result in λ^sub max^-values ~340 nm for the unfolded states
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The three-dimensional structure of BChE is very similar (21)
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Conformational exchange is present when the difference between the R^sub 2^ rates measured with δ = 3
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Although DNA cannot be condensed by monocations in aqueous media (Wilson and Bloomfield, 1979), in a crowded environment DNA condensation is promoted by an increase in the concentration of monocations (Livolant and Amelie, 1996)
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Apolipoprotein A-II regulates HDL stability and affects hepatic lipase association and activity
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The AFM images of the mature amyloid at 70°C (Fig
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25 µg/ml), selenous acid (6
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. the high mannose GlcNAc^sub 2^Man^sub 9^ glycan chain was attached with Asn-75 (Nδ2) of the protein (Protein Data Bank code 2SBA) using MOE (Molecular Operating Environment) software, and energy was minimized using a force field of AMBER99
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. Our results show that the secondary structure of EI^sup sc^ was not substantially altered, as suggested by FTIR experiments, but quenching experiments and proteolysis patterns suggest that there were small conformational changes upon binding of PEP; in addition, the thermal stability of EI^sup sc^ was slightly modified when any effector was added
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. the enzyme was more resistant to proteolysis (open and solid circles) (Fig
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. The fact that for NDSB samples the obtained Δλ^sub max^-value between the folded and the unfolded state is much larger as compared to that measured with pure buffer samples is a further indication that aggregates reduce the magnitude of red shifts
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. Control samples (freshly cleaved mica, mica, and aqueous HCl-solution) were also investigated with AFM to exclude possible artifacts
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. Extended and docked conformations of the hairpin ribozyme were also resolved and quantified in previous ensemble trFRET experiments (N
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