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. A Gaussian-chain model for treating residual charge-charge interactions in the unfolded state of proteins
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. Solution structure of the 40,000 Mr phosphor) 1 transfer complex between the N-terminal domain of enzyme 1 and HPr
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. This extraction procedure was repeated twice
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. 1, the optimal temperature of ACYI activity was ~65°C, which is consistent with the previous observations (25,26) when considering that different heat treatments were used
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. RESULTS Biophysical characterization of the rSBA The biophysical characteristics of the rSBA are very much similar to that of the native SBA obtained from the seeds of Glycine max
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. Rather solvent accessible tryptophan residues in the folded state (Fig
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. Besides, as these globules have no germinal vesicle and do not stain for DNA, they cannot be considered realistic model cells
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.We thank Janny Hof for critical reading of the manuscript
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. Aliquots of the aggregation reaction were withdrawn and mixed with an assay buffer (10 mM HCl, containing a final concentration of ANS, Bis-ANS, or DCVJ at 2
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. The EM data show that disk incubation in high Gdm HCl concentrations leads to a small but significant increase in the particle diameter (Fig
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. The advantage of the hypergutter approach, however, is that it identifies diffusive subspaces in cases where the standard diffusion-collision model does not
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. A rather smooth continuation of the configurational entropy buildup profile without observable change or perturbation, after appending a second trajectory that has a large conformational overlap with the first one (Fig
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. These hybridomas were cloned by the limiting dilution method, and finally three clones producing monoclonal antibodies reactive with P
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