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It is worth noting that a multi-event equilibrium unfolding/ dissociation of human GR by GdnHCl has been previously reported (Nordhoff et al
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Natural selection, though, exercises quality control over genes
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The sample with the protein and the effectors was incubated for a minimum of 2 h at room temperature
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fluorescent residues are involved in the self-association interface: and, 3), the value of 1 µM could be considered an upper estimation of the EI^sup sc^ self-dissociation constant
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The dependence of the ANS fluorescence intensity, in ice at - 13°C, on the starting ANS concentration in solution (10-200 µM) is shown in Fig
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The availability of intracellular spectra opens a new window for the investigation of intracellular processes, since the spatial and temporal fingerprints of nonwater molecules, be they physiological cellular components or external substances, can directly be followed on a subcellular scale
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Calmodulin and Signal Transduction
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Of course, activity studies must be done to verify this idea
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To facilitate direct comparison of the CD and DSC data in Fig
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6 c, two highly-twisted fibrils with different morphologies overlapping each other
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10), as well as an upper limit on the intrinsic statistical broadening of the FRET histograms due to shot noise (Deniz et al
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5 ml papain digest buffer (Sodium citrate (55 mM), Sodium chloride (150 mM), Cysteine Hydrochloride (5 mM), EDTA (5 mM) (all Merck, Poole, UK)); and 1 µg/ml papain (Sigma, Poole, UK)
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05 was used for the independent t-test and ANOVA
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