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. The thermal unfolding of ACYI might be a three-stale process involving an intermediate appearing at ~68°C
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. Secreted DmAChE was purified to homogeneity using the following steps: 70% ammonium sulfate precipitation, dialysis, affinity chromatographies on a procainamide-Sepharose column and on nitrilotriacetic acid-nickel agarose (39)
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. Figure 4a for the NC flux reveals that there is no significant increase in wetting force for Sn-Pb solder at the temperatures 255°C and 275°C, whereas the maximum wetting force increases slightly from 2
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. 5-ns internal motion in the CaM:SEF2-1mp complex can be attributed to motions of the separate N- and C-terminal domains of CaM, because the free domains have an overall correlation time of ~3 ns
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. Increased protein backbone conformational entropy upon hydrophobic ligand binding
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. Protein transfer between A-I-containing lipoprotein subpopulations: evidence of non-transferable A-I in particles with A-II
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. This illustrates case B (Fig
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. For the higher dissolving temperatures, the polymer appeared to be dissolved well before the end of the 2 hours
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. 75# 74#Note: period-specific average monthly rates relativeto rates in the pre-RP period indicated with #
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. For example, at 20 µm ANS where binding sites are fully saturated in solution and the intensity has reached its maximum value, the corresponding intensity in ice is merely 3%-5% of the value in the liquid water
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. The data establish that every protein exhibits enhanced ANS fluorescence and therefore significant ice-induced ANS binding
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. Table 2 shows the required gradient field strengths for the respective voxel size and rf bandwidth
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. Among the various methods that can provide wettability information are area of spread tests, edge dip and capillary rise test, globule test method, rotary dip test, wetting balance test, and microwetting balance test
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. This is believed to assist the target-specific deformation of the binding sites in CaM that is necessary for productive binding
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. For example, sodium ion, which has been reported to have the lowest DNA binding potential, is the most effective monocation for DNA compaction
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